Paraffin in the embedding center should be kept:

Tissue specimens with layers, such as a GI biopsy, should be embedded .

Examination of a section of H&E stained skin shows cells that appear to have shrunk; there is a lot of artificial spaces surrounding the cells. The best possible solutions include:

During frozen-sectioning, you notice the sections are incomplete. Which action(s) should be taken to prevent this artifact?Check all that apply

Match the following chemical with its property. Answers can be used more than once, choose the BEST answer.

A tissue ribbon picked up from this waterbath that has yet to be skimmed (cleaned) might be expected to show? Microtomy2.jpg

If using phosphate-buffered formaldehyde solutions for fixation, the phosphate salts will precipitate out if the concentration of the initial alcohol is greater that .

What type of microtome does the cryostat usually contain?

What is wrong (in regards to embedding) in this picture? embed2.jpg

What is the necessary corrective action? Microtomy3.jpg