Which of the following is FALSE regarding purifying proteins using the ammonium sulfate precipitation?
You wish to express a protein expression system that is very…
You wish to express a protein expression system that is very fast, inexpensive, and has established protocols that have been perfected over the past few decades, rather than explore a newer system. Which expression system would you choose?
Why are proteins stored in a buffer with dithiothreitol or β…
Why are proteins stored in a buffer with dithiothreitol or β-mercaptoethanol?
You purify a protein with an anion exchange chromatography a…
You purify a protein with an anion exchange chromatography and elute it with 1 M NaCl. You take this eluate and load it directly onto a cation exchange column, but the protein was found only in the flowthrough and not the elution. What was the cause of this?
A pregnancy test is equivalent to which of the following tec…
A pregnancy test is equivalent to which of the following techniques?
You are working with a protein that is polyubiquitinated (a…
You are working with a protein that is polyubiquitinated (a variable number of ubiquitins), which you visualize with an anti-ubiquitin antibody. You treat the protein with an enzyme that cleaves ubiquitin from proteins and degrades it (+ lane). What would be the expected results?
A blue native PAGE is advantageous over a standard native ge…
A blue native PAGE is advantageous over a standard native gel because it
You’re working with an MBP-p53 fusion protein, which is link…
You’re working with an MBP-p53 fusion protein, which is linked by covalent peptide bonds. You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.
A protein has a pI of 5, and is in a buffer at pH 7. What ty…
A protein has a pI of 5, and is in a buffer at pH 7. What type of column should it bind to?
Which of the following would be best to study the glycosylat…
Which of the following would be best to study the glycosylation state of a protein?