Which technique uses phenyl isothiocyanate to bind to N-terminal amino acids?
In cation exchange chromatography, when the salt gradient is…
In cation exchange chromatography, when the salt gradient is increased from 0 to 100%,
When running size exclusion chromatography, you get two peak…
When running size exclusion chromatography, you get two peaks merged together as seen below. Which of the following is the most appropriate solution for separating the two peaks?
In western blotting, the enzyme is typically conjugated to t…
In western blotting, the enzyme is typically conjugated to the
The image below, which illustrates an increased size on the…
The image below, which illustrates an increased size on the gel after the addition of Protein 2, is demonstrating
You perform an SDS-PAGE and observe a band at the expected m…
You perform an SDS-PAGE and observe a band at the expected molecular weight of your target protein. What technique would be best to confirm this?
Which of the following polyacrylamide gel reagents is NOT pr…
Which of the following polyacrylamide gel reagents is NOT properly matched up with the correct function?
Ammonium sulfate precipitation
Ammonium sulfate precipitation
Why would the diameter of the beads affect SEC resolution?
Why would the diameter of the beads affect SEC resolution?
You wish to express a human protein that is properly folded,…
You wish to express a human protein that is properly folded, contains cleaved signal peptides, proper glycosylation, and other processing modifications. Which expression system would you choose?