Which statement about chromatography is TRUE?
In which expression system does E. coli RNA polymerase enzym…
In which expression system does E. coli RNA polymerase enzyme transcribe the gene of interest on the plasmid into mRNA?
Which is TRUE regarding ELISA?
Which is TRUE regarding ELISA?
Which method would be best for purifying soluble proteins?
Which method would be best for purifying soluble proteins?
You run an enzyme through gas chromatography. You would expe…
You run an enzyme through gas chromatography. You would expect to see
SDS-PAGE requires sodium dodecyl sulfate to be added to the…
SDS-PAGE requires sodium dodecyl sulfate to be added to the sample because it
If you inject a human protein into a goat, the antibodies ma…
If you inject a human protein into a goat, the antibodies made against the human protein will be
An experiment was performed to induce and purify the protein…
An experiment was performed to induce and purify the protein X. In the gel below, Lane 1 shows non-induced cells; Lane 2 shows induced cells; Lane 3 is purified MBP-X; Lane 4 represents the protein after TEV cleavage; Lane 5 represents the flowthrough after the cleaved sample has been run through an amylose column; Lane 6 represents the purified TEV protease. What is the molecular weight of the free MBP tag?
You are reading a paper which shows an image of a gel, and c…
You are reading a paper which shows an image of a gel, and concludes that protein A is expressed in higher amounts than protein B based on band intensity. You could confirm this by using the
Which is TRUE regarding SDS-PAGE and western blotting?
Which is TRUE regarding SDS-PAGE and western blotting?