Table 3: Effects of Enzyme Concentration

Part 3: Effect of Enzyme Concentration in Enzyme Activity  At a constant enzyme concentration and at lower concentrations of substrates, substrate concentration is the limiting factor. As the substrate concentration increases, the enzyme reaction rate increases. However, at very high substrate concentrations, the enzymes become saturated with substrate and a higher concentration of substrate does not increase the reaction rate. 

Conclusion: Answer the following questions for Part 4: Effect of pH in Enzyme Activity.

Explain your results for Test tube 1. How did the temperature influence the enzyme activity?

Explain your results for Test tube E2. How did the enzyme concentration influence the height of bubbles that were produced?

Explain your results for Test tube 3. How did the temperature influence the enzyme activity?

What happened when H2O2 was added to the potato in test tube A?

Explain your results for Test tube 2. How did the temperature influence the enzyme activity?

Explain your results for Test tube P1. How did the pH influence the enzyme activity and its production of bubbles?

Materials:  Three clean test tubes  Four beakers  Two 5 mL Graduated pipet   Hydrogen peroxide (H2O2)  Catalase (potato)  Ruler  Wax pencil  Procedure:  In order to test enzyme concentration, we will be diluting the catalase with water. Use the beakers to dilute the catalase. Mix according to the table below.  Beaker  Water  Catalase  Concentration  1  3 mL of water  1 mL of Catalase  25%  2  2 mL of water  2 mL of Catalase  50%  3  1 mL of water  3 mL of Catalase  75%  4  0 mL of water  4 mL of Catalase  100%  Label each of your test tubes (E1, E2, E3, and E4).  To each of your test tubes add 4 mL of hydrogen peroxide.   To E1, add 1 mL of 25% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  To E2, add 1 mL of 50% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  To E3, add 1 mL of 75% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  To E4, add 1 mL of 100% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  Results: (Watch the Lab 7. Enzymes video)  Table 3: Effects of Enzyme Concentration (Copy and paste the table to answer Question 18 and fill it in with your data) Test Tube  Catalase Concentration  Bubble Height (mm)  E1      E2      E3      E4