You can search for proteins that bind DNA using all of the following EXCEPT
Which of the following is the best technique used for lysing…
Which of the following is the best technique used for lysing cells?
Below is an SDS-PAGE (A) of an induced lysate (Lane 2) and a…
Below is an SDS-PAGE (A) of an induced lysate (Lane 2) and a western blot (B) using an anti-target protein antibody. The protein was purified via affinity chromatography (Lanes 4-6). What is the molecular weight of the major contaminant (not the target protein) that was removed by the purification?
Heat shock proteins were discovered by comparing protein exp…
Heat shock proteins were discovered by comparing protein expression levels in cells at different temperatures using
What conclusions could you draw from this peak from a size e…
What conclusions could you draw from this peak from a size exclusion column?
A protein was run on both an SDS-PAGE and native gel, as sho…
A protein was run on both an SDS-PAGE and native gel, as shown below, with the addition of a drug (+) that disrupts protein structure. Which of the following is TRUE?
Which of the following can be used to determine a protein’s…
Which of the following can be used to determine a protein’s molecular weight, or to detect protein interactions with metals by analyzing weight differences in the holo vs. apo state?
Which would be the best choice for studying protein-protein…
Which would be the best choice for studying protein-protein interactions or protein modifications?
Which of the following techniques can be used to identify wh…
Which of the following techniques can be used to identify where a protein binds in a DNA sequence?
What is the purpose of Tween-20 in the western blot washes?
What is the purpose of Tween-20 in the western blot washes?