Match the following reagent with its function. Answers may be used more that once.

Tissue sections, stained with H&E, appear to have cloudy uneven. Plausible explanations include:Check all that apply

Describe a circumstance why it may be necessary to surface decalcify a section of tissue. How would this be accomplished?

Compare the photographs (left-initial result / right-final product). The spleen tissue on the left was overdehydrated/processed. What corrective action step was most likely taken to produce the outcome that is seen on the right? Microtomy9.jpg

Which of the following fixatives will render bone biopsies radiopaque and therefore prevent x-rays from being used to determine the end point of decalcification?Check all that apply

Paraffin in the embedding center should be kept:

Tissue specimens with layers, such as a GI biopsy, should be embedded .

Examination of a section of H&E stained skin shows cells that appear to have shrunk; there is a lot of artificial spaces surrounding the cells. The best possible solutions include:

During frozen-sectioning, you notice the sections are incomplete. Which action(s) should be taken to prevent this artifact?Check all that apply

Match the following chemical with its property. Answers can be used more than once, choose the BEST answer.