Subjuntivo: Lee el problema siguiente y escribe tus recomendaciones para soluciones. Usa el subjuntivo y los verbos o frases siguientes:    Problema:    Todos sabemos que el calentamiento global está afectando el medio ambiente de una manera negativa. Necesitamos soluciones para evitar más calentamiento global y proteger  el medio ambiente mejor.  Frases para usar: recomendar, sugerir, es bueno que, es mejor que, es necesario que, es urgente que, es probable, no creer 1. 2. 3. 4. 5. 6. 7. 8.

Preguntas escritas:  Escriba la respuesta con una frase completa. Use los pronombres de objeto directo/indirecto cuando sea posible. (28) Write an answer out in Spanish- do your best using the pronouns for direct and indirect objects. Careful with 7.! (Don’t force the pronouns if there aren’t any!) (á  é  í  ó  ú ñ ¡¿)  ¿Tú les prestaste dinero a tus amigos la semana pasada?  ¿Tu profesora te regaló puntos extras?  ¿Le lavaste la ropa a tu compañero de cuarto o a tu hermano la semana pasada? ¿Tu compañero de cuarto (o un amigo) te pidió un favor la semana pasada?   ¿Sus padres les dicen “te quiero mucho” a Ustedes?   ¿Sus profesores les escribieron correos electrónicos a ustedes esta semana?   ¿Fuiste a las montañas con tus amigos el fin de semana pasado?

When an ELISA test  was being performed, the technician forgot to add the enzyme-conjugated secondary antibody during the assay.  Under such circumstances, a negative results from the sample would be considered as

Cells that have NOT undergone transformation with pGFP should be killed after plating  on LB-AMP.  

The following gel shows the results of a crime scene investigation.  Lane 1 shows a DNA sample that was obtained from the scene of a crime (there was evidence of the criminal cutting himself, so it’s the criminal’s blood sample that produced the DNA bands in lane 1). Lanes 2-7 shows a DNA sample from six potential suspects held in connection with the crime.  Which suspect (#2 through #7) is most likely to have committed the crime?

A fragment of DNA that is 3000 bases long will run farther than a 1000-bp long fragment during gel electrophoresis.

During a PCR reaction in progress, the PCR machine malfunctions and therefore cannot not maintain a temperature of 95oC.  Name the step of the PCR process that will be directly affected by this problem.

The following gel shows the results of a crime scene investigation.  Lane 1 shows a DNA sample that was obtained from the scene of a crime (there was evidence of the criminal cutting himself, so it’s the criminal’s blood sample that produced the DNA bands in lane 1).  Lanes 2-7 shows a DNA sample from six potential suspects held in connection with the crime.  Arrange the bands labeled A, B, C, D from largest to smallest.

In the transformation experiment performed, the GFP gene confers resistance to the antibiotic Ampicillin.

During transformation of bacteria with pGFP, any bacteria that have not taken up the pGFP plasmid will not grow on which of the following plates?