Describe the key steps in a western blot experiment (6 points). When comparing relative quantitative difference of a target protein between two samples, scientists often include a western blot on a housekeeping protein, why? (2 protein).

Scientists have engineered the cas9 protein in CRISPR to “Cas9 nickase (Cas9n)” and “dead Cas9 (dCas9)”. Describe how they differ from the original cas9, and the utilities of these two engineered enzymes. 

Math166_HF02_Test2.pdfAbove is a link to the pdf of the test. In the event you cannot access it, please let me know! -Prof Matt

You are developing an exercise program for a client who has some anxiety-related concerns. One point of consideration is the intensity of his exercise program. Which of the following are correct statements?  This is a multiple answer question. At least one response is correct.  Select any number of answer(s) you believe to be correct. 

Anxiety is a relatively complex mental health condition with many characteristics. Class lecture provided which of the following descriptions of anxiety? This is a multiple answer question. At least one response is correct.  Select any number of answer(s) you believe to be correct. 

Competent cells can be made by two major different methods: and .

What is a potential complication of untreated gonorrhoea for this patient?

In the CRISPR system, what is the key difference between cas1 and cas9 proteins? 

In Western blot, indirect detection using an unlabeled primary antibody followed by a labelled secondary antibodies increases the detection sensitivity.

Which of Miss C’s presenting complaints most strongly suggests an alternative diagnosis to urinary tract infection?