Which of Miss C’s presenting complaints most strongly suggests an alternative diagnosis to urinary tract infection?

Sequencing a genome of 200Mb, using the Illumina paired-end 100bp, and yielding 50 million reads, gives a coverage of:

Theme: Steroids Prednisolone can be stopped abruptly Prednisolone dose should be temporarily doubled Prednisolone dose should be temporarily halved Prednisolone dose should be tapered up slowly Prednisolone dose should be tapered down slowly Hydrocortisone IV should be given Hydrocortisone topical cream should be given Hydrocortisone oral tablets should be given   For each of the possible scenarios described in the following 3 questions, you have been asked to give advice with regards to steroid treatment. In these scenarios, Miss C is no longer pregnant or breastfeeding.  Select the most appropriate regimen from the options above. Each option may be used once, more than once or not at all.

Mr A informs you that he participates in Ramadan. Utilising the following resource, what is the most appropriate advice to provide to Mr A regarding his medications during this period? https://www.england.nhs.uk/midlands/wp-content/uploads/sites/46/2020/11/SWBCCG-Summary-Guidance-Diabetes-Ramadan-V1.pdf

Competent cells can be made by two major different methods: and .

In single-cell RNAseq analysis, what is the use of Unique Molecular Identifier (UMI)?  

What is a potential complication of untreated gonorrhoea for this patient?

Several years later, Miss C is thinking about trying to start a family. She sees her GP, who arranges thyroid function tests.   Patient Result Reference Range Free T4                   60 mmol/L (70-140 mmol/L) TSH                       9 mIU/L (0.3-4.7 mIU/L)

Which of the following is the most appropriate advice to provide to Mr A relating to the use and storage of Ozempic? https://www.medicines.org.uk/emc/product/9748

John collected 9 samples of nematodes treated with different chemicals. He planned to examine differences among their transcriptomes by Illumina sequencing. However, he forgot to include index sequences for his library preparation in the single sequencing run for multiplexing. What problem will John face?