You need to make 100mL of TE pH 8.0 buffer (consisting of 120mM Tris and 1mM EDTA) for a DNA extraction. You have Tris base powder and EDTA powder from which to make it. Tris powder has a MW of 121g/mol, while EDTA powder has a MW of 292g/ml. How much Tris powder will you need for this desired volume of TE solution? How much EDTA powder will you need?
To make 1 liter of 5X SSC, mix together [answer 1] ml of 20X…
To make 1 liter of 5X SSC, mix together ml of 20X SSC and ml of ddH2O
You extracted RNA using the organic solvent method (Trizol,…
You extracted RNA using the organic solvent method (Trizol, which uses phenol and chloroform to extract this molecule). You measured your extracted sample and the A260/A280 ratio is 1.7. You would describe this preparation as:
Which of the following is NOT involved in gene expression (i…
Which of the following is NOT involved in gene expression (i.e. transcription and translation)?
What is the purpose of diethylpyrocarbonate (DEPC) in nuclei…
What is the purpose of diethylpyrocarbonate (DEPC) in nucleic acid extraction protocols?
What is the purpose of SDS in non-organic nucleic acid extra…
What is the purpose of SDS in non-organic nucleic acid extractions?
You need 250 mL of 0.25X tris-acetate-EDTA (TAE) for the buf…
You need 250 mL of 0.25X tris-acetate-EDTA (TAE) for the buffer tank to run an agarose slab gel. You have 50X stock TAE. How much of the stock TAE do you need to dilute to 250mL?
In a two digit whole number the second column from the right…
In a two digit whole number the second column from the right in the number represents units.
Subtract the following numbers to obtain the difference:3,97…
Subtract the following numbers to obtain the difference:3,972 – 1,028 =
Two rooms needs 42 feet of electric baseboard heaters. Lengt…
Two rooms needs 42 feet of electric baseboard heaters. Lengths of 9 feet, 8 feet, 7 feet, and 7 feet are already placed. What length of baseboard heater in feet still must be placed?