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9. OPTICAL TRAPS  To further understand interactions between HERKY3’s downstream targets, you useoptical traps to measure the interaction forces between two proteins. a. (1 pt) In your calibration measurements you find that the bead stiffness is toohigh and that you are losing sensitivity. What is one way in which the beadstiffness can be reduced so that your measurements become more sensitive? b. (1 pt) True/False. Optical trapping is only appropriate on subcellular scales (i.e.,purified proteins and structures). c. (3 pts) Which force measurement mode is most appropriate to characterizemovement of a translocating molecule, such as a molecular motor? Brieflyexplain the principle of this mode.

Olivia repeatedly grabs toys from her sibling while they’re playing together. To address this, her mom leads her out of the playroom and has her wait quietly in the kitchen for a brief period. As a result, Olivia’s behavior of grabbing toys decreases over time. Which negative punishment procedure is Olivia’s mom using in this scenario?

Which of the following best describes existing cache response cost?

Which of the following best describes response interruption and redirection as an intervention technique?

3. MICROSCOPY a. (2 pt) You want to image two fluorescent proteins within the same sample.mEGFP:Excitation max: 488nmEmission max: 507nmmCherry2:Excitation max: 589nmEmission max: 610nmAssume LEDs are used for excitation that precisely match the excitation maxima. Which type of filter would be best to use as an emission filter to detectmCherry2? a. Longpass filter with a 50% cut-off at 520nmb. Shortpass filter with a 50% cut-off at 600nmc. Bandpass filter with 50% cut-offs at 520nm and 660nmd. Bandpass filter with 50% cut-offs at 595nm and 625nm   b. (3 pts) Briefly describe the components of a filter cube and their purposes.

Which of the following is NOT an error to avoid when implementing time-out as a behavioral intervention?”

During a group game, a child begins aggressively grabbing objects from other children. To prevent harm, the teacher gently holds the child’s hands in their lap until they calm down. Which positive punishment procedure is being applied?

The ______________ muscle is essential for overhead activities such as washing your hair during bathing 

When using response interruption and redirection (RIRD), which of the following steps are typically included in the procedure?