A 20 year old athlete with a history of moderate persistent…

A 20 year old athlete with a history of moderate persistent asthma. He present to the asthma clinic and relates decreasing exercise tolerance, increased nighttime cough, increased use of his SABA and a moderate reduction in PEFR values over the past 14 days. Which of the following best explains his primary problem?

You set up an in vitro translation system containing the ent…

You set up an in vitro translation system containing the entire translation machinery but missing any component of the endoplasmic reticulum (ER) targeting machinery. To this system, you can add mRNAs encoding either a 20 kDa secretory protein or a 20 kDa cytosolic protein. You perform in vitro translation in the presence of radioactively labeled methionine, with or without the addition of saturating amounts of SRP or ER-derived microsomes, as indicated below. After separating the protein products by SDS-PAGE, and visualizing the radioactivity by autoradiography, you obtain the below results. The presence or absence of each component in the reaction is indicated at the top of the corresponding lane(s) by + and –, respectively. The numbers on the left indicate the apparent molecular mass (×1000) of spots on the gel. Which is the secretory protein ? Which of the reactions omitted SRP ?