12. 13. 14. 15. 16. 17. 18. 19. 20.

Metabolism Upload an image of your drawing and explanation. Show how a molecule of palmitic acid (16:0) that has entered a muscle cell is “tagged” or prepared for

Upload an image of your drawings. Outline the steps necessary to convert the final odd-chain fatty acid

Upload your answers to these questions. a.) Draw out the synthesis of the ketone body, acetoacetate, which forms in the liver under fasting conditions. It is formed in three steps.  (4 pts.) b) For the the last step in the synthesis of acetoacetate, include the arrow pushing mechanism for the reaction and identify which reaction in glycolysis it is parallel to. (3 pts.) c.) Draw out the reactions that converts acetoacetate into the other two ketone bodies. Show the structures the three ketone bodies and label each with an acceptable name.  (2 pts.) d.) Ketone bodies are exported from the liver and utilized as an energy source by the extra-hepatic tissues. The brain, which normally utilizes glucose as its energy source, can, under fasting conditions, partially convert to using ketone bodies over the course of a few days. Show how a molecule of the ketone body, 

Electron Transport Chain and Oxidative Phosphorylation  Write out the missing compound, cofactor, name, element, or ion for each of the 24 points (numbered circles) on this diagram of the electron transport chain.    1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.   20. 21. 22. 23. 24.

Upload your answers to these questions. a.) Outline the reactions required to release a molecule of stored glucose from liver glycogen into the bloodstream. You may use acceptable abbreviations for the names of compounds–structures are not necessary. Include enzyme names or general type of enzymatic reaction for each step. (3 pts.) b.) Again in the liver, outline the series of reactions required to store a molecule of glucose as glycogen. You may use acceptable abbreviations for the names of compounds-–structures are not necessary. Include enzyme names or general type of enzymatic reaction for each step.  (3 pts.) c.) Briefly explain what provides the free energy required for the reactions outlined in part b.  (2 pts.)

Identify the eight following cofactors and coenzymes and, from the metabolic reactions we have encountered, name a reaction that requires the cofactor. Name or acceptable abbreviation is ok. (1 pt. each)   Name:                                      Rection:   c.         Name:                                     Reaction: d. By NEUROtiker – Own work, Public Domain, https://commons.wikimedia.org/w/index.php?curid=1555660 Name:                                    Reaction:

Upload an image of your answers to this question. Draw the following structures: Oleamide, the simple amide (NH2) of oleic acid has been isolated from the spinal fluid of cats and identified as the molecule responsible for causing sleep. In humans, oleoyl ethanolamide is an endogenous regulator of food intake produced in the intestines and has potential as an anti-obesity drug. Draw the structure of oleic acid. (2 pts.) Anandamide functions as an endogenous ligand of cannabinoid (psychoactive compounds in marijuana) receptors in humans and gets its name from the Sanskrit word, “ananda”, meaning bliss. This compound is the ethanolamine (HO-CH2-CH2-NH2) amide of arachidonic acid. Draw the structure of anandamide. (3 pts.) Saplenic acid 16:1(D10) is the most abundant lipid component of human sebum. Draw the structure of this fatty acid. (2 pts.) Phosphtidylserine is a regulator of apoptosis (programmed cell death, causing a cell to commit “suicide”) in response to calcium-dependent stimuli. Draw a phosphatidylserine of your choice with two different unsaturated fatty acids and give the names of the acids you chose to draw. (3 pts.) Ceramide-1-phosphate is a potent inhibitor of apoptosis or programmed cell death, as well as an inducer of cell survival. Draw the ceramide that has a linolenic acid attached and a phosphate attached at the terminal, 1 position. (3 pts.) A very long chain polyunsaturated fatty acid (VLC-PUFA) is found in a glycerophopholipid associated with the retinal membrane in the eye. This is a phosphtidyl choline compound with DHA (docosahexenoic acid – C-22:6 (D4….) esterified at the 1 position of the glycerol and a C-34:6

Upload an image of your answer to this question. Both pyruvate decarboxylase (an enzyme associated with alcoholic fermentation) and one of the enzymes in the pyruvate dehydrogenase complex (E1) require thiamine pyrophosphate (TPP).  In fact, the substrate goes through common carbanion intermediate in both of these enzymatic reactions. a. Outline the mechanism for the formation of this common resonance stabilized carbanion intermediate starting with pyruvate and show how this carbanion intermediate is resonance stabilized.  For TPP, you only have to show the structural part that is involved in the reaction (the business end of the cofactor).  (3 pts.) b). Beginning with the common stabilized carbanion anion intermediate, show how acetaldehyde is formed as the product of pyruvate decarboxylase in alcoholic fermentation and how a covalent S-acetyl dihydrolipoyl (the 2-C piece attached to the lipoic acid) intermediate is formed in the pyruvate dehydrogenase complex.  For TPP and lipoic acid, you only have to show the ring structural part that is involved in the reaction (the business end of the cofactor).  (3 pts.)

Upload an image of your answer to this question.  There are two cofactors, lipoic acid (the crane), FAD, which are alternately reduced and oxidized in the pyruvate dehydrogenase complex, and NAD+, which is reduced as a final product. Draw the structures of the reduced forms of each of these three cofactors (do not include the Cys amino acid residues associated with the protein). You only need to show the relevant structural ring parts that change when each of these are reduced. (6 pts.)     Reduced form of Lipoic acid         Reduced form of FAD                Reduced form of  NAD+