Materials:  Three clean test tubes  Four beakers  Two 5 mL…

Materials:  Three clean test tubes  Four beakers  Two 5 mL Graduated pipet   Hydrogen peroxide (H2O2)  Catalase (potato)  Ruler  Wax pencil  Procedure:  In order to test enzyme concentration, we will be diluting the catalase with water. Use the beakers to dilute the catalase. Mix according to the table below.  Beaker  Water  Catalase  Concentration  1  3 mL of water  1 mL of Catalase  25%  2  2 mL of water  2 mL of Catalase  50%  3  1 mL of water  3 mL of Catalase  75%  4  0 mL of water  4 mL of Catalase  100%  Label each of your test tubes (E1, E2, E3, and E4).  To each of your test tubes add 4 mL of hydrogen peroxide.   To E1, add 1 mL of 25% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  To E2, add 1 mL of 50% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  To E3, add 1 mL of 75% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  To E4, add 1 mL of 100% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3.  Results: (Watch the Lab 7. Enzymes video)  Table 3: Effects of Enzyme Concentration (Copy and paste the table to answer Question 18 and fill it in with your data) Test Tube  Catalase Concentration  Bubble Height (mm)  E1      E2      E3      E4     
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