Given Bx resource Table 1, the coupled reaction catalyzed by hexokinase, and its uncatalyzed half-reactions, select the answer that best describes the sign of the ∆Go’ of each. coupled reaction:    g l u cos e   +   A T P → g l u cos e ‐ 6   p h o s p h a t e   +   A D P half-reaction 1:        g l u cos e   +   P i → g l o cos e ‐ 6   p h o s p h a t e   +   H 2 O half-reaction 2:        A T P   +   H 2 O   →   A D P   +   P i

Brown adipose tissue generates heat by controlled dissipation of the proton gradient across the inner mitochondrial membrane through the mitochondrial uncoupling protein (UCP). Identify the effect on mitochondrial properties that are associated with increased uncoupling protein activity. Answer choices: Decrease Increase No change    Parameter Effect of increased uncoupling protein activity    Intermembrane space pH    Matrix pH    ATP synthase activity    ATP/ADP translocase    

Isolated mitochondria are able to sustain ATP synthesis when provided with ADP, Pi, O2, and a regenerating supply of NADH or FADH2 as the sole source of electrons for oxidative phosphorylation. Predict how inhibition of Complex I will affect O2 consumption and ATP synthesis. Answer choices: Decrease Increase No change Site of inhibition Sole source of electrons Oxygen consumption ATP synthesis Complex I NADH Complex I FADH2

Given the metabolic map below, determine the net change in metabolic processes as a consequence of increased energy status. CitricAcidCycleRegulation.png 

Given the definition of a monosaccharide as an aldehyde or ketone having two or more hydroxyl groups, identify the monosaccharide among the glycolytic intermediates below.  glycolytic 3-carbon intermediates.png 

Given that all phosphotransferases have similar catalytic mechanisms, consider the half-reactions for the uncatalyzed transfer of inorganic phosphate from 1,3-BPG to ADP. One half-reaction produces water and the other consumes water. Observe that the phosphoglycerate kinase (PGK)-catalyzed phosphoryl transfer from 1,3-BPG to ADP does not include water. Select the best explanation for why the catalyzed reaction does not include water. uncatalyzed half-reaction 1 1,3-BPG + H2O → 3BPG + Pi uncatalyzed half-reaction 2 ADP + Pi → ATP + H2O PGK-catalyzed coupled reaction 1,3-BPG + ADP → 3PG + ATP

Given the Bx resource and the reductants below, identify the one that will most readily give up its electrons.

The exergonic flow of electrons from NADH catalyzed by Complex I is sufficient to drive which of the following endergonic processes? 

Given the Gibbs free energy equation in the Bx resource, the reaction catalyzed by phosphoglycerate kinase, its ΔGo’, and its ΔG under typical physiological conditions, 1 , 3 – B P G + A D P ⇄ 3 P G + A T P Δ G 0 ‘ = 18 . 8   k J · m o l – 1 Δ G = 1 . 3   k J · m o l – 1 identify each statement as True or False.   The reaction is near equilibrium under physiological conditions.   ΔG is the determining factor for whether the reaction is near or far from equilibrium.   Elevating the concentration of ATP will accelerate the forward reaction.   At equilibrium, the product of the concentrations of 3PG and ATP will equal the product of the concentrations of 1,3BPG and ADP.

Malate dehydrogenase catalyzes the following reaction in the citric acid cycle (ΔGo’= +29.7 kJ mol-1).malate + NAD+ → oxaloacetate + NADHMatch each half reaction with its correct thermodynamic nature of the reaction.