In the procedure, the authors state that the purified protein was S-alkylated, meaning that a saturated carbon was attached to an S atom on the protein of interest before it is subjected to further purification by HPLC. Briefly explain why it is desirable to treat the reduced protein with iodoacetic acid (ICH2CO2H) before HPLC. 

A random sample of car ads were studied and the horsepower​ and car’s selling price were collected. ​You have obtained the graph and regression equation below to determine if the horsepower can be used to predict a car’s selling price. Car Price = 3643 + 67.91(Horsepower) What does the slope of the line indicate?

Treatment of a polypeptide with cyanogen bromide (chemically selectively cleaves peptide bonds on the carboxy side of Met residues) followed by Edman degradation of each of these fragments, gave these four fragment sequences. 1. Asp-Ile-Lys-Gln-Met, 2. Lys, 3. Lys-Phe-Ala-Met, 4. Tyr-Arg-Gly-Met  Trypsin hydrolysis of the same polypeptide followed by Edman degradation of each fragment gave these four sequences. 1. Gln-Met-Lys, 2. Gly-Met-Asp-Ile-Lys, 3. Phe-Ala-Met-Lys, 4. Tyr-Arg  Based on this information, write out the sequence of the peptide.

Pyruvate may also be converted to an amino acid in a single step. Give each of the following: the three-letter code or the name of the amino acid formed in this reaction a generalized enzyme name that describes this reaction type the names or acceptable abbreviations for any necessary cofactors for the reaction

Fill in the blank: If the Pearson correlation value (r) is negative then the _______is also negative.

 Questions 14 – 17 will refer to the same research scenario. A delivery company has asked a random sample of delivery drivers to report the number of miles driven on Wednesday 8/6/2025. The data was summarized with the following box plot. How would you describe the shape of the distribution?

Well-fed State Insulin Questions 1–11 Image Description A diagram illustrating metabolic pathways during the well-fed state, characterized by prevalent insulin levels, with a focus on glycolysis, glycogen synthesis, and the citric acid cycle.Molecule 1: Required and produced in the reaction where glucose (Glc) is phosphorylated to glucose-6-phosphate.Molecule 2: Enzyme catalyzing the conversion of glucose 6-phosphate to glucose 1-phosphate. Molecule 3: Reactant reacting with glucose 1-phosphate, resulting in the production of molecule 4 as a byproduct and molecule 5 as the main product. Molecule 5: Converted into glycogen by enzyme numbered 6, with molecule 7 produced as a byproduct. Molecule 8: Sub-reaction occurring during the conversion of malate into oxaloacetate.Molecule 9: Molecule added to pyruvate when pyruvate is converted to acetyl-CoA, releasing molecule 11, with sub-reaction 10 occurring simultaneously. UTP or UDP or PLP (vitamin B6) ATP/ADP or biotin ATP/AMP or phosphatase or fumarate GTP/GDP or kinase or glutamate NAD+/NADH or dehydrogenase or  α -ketoglutarate NADP+/NADPH or transaminase or carnitine FAD/FADH2 or Mutase or CO2 or HCO3- Pi or 2Pi (phosphate) or synthase or citrate H2O or urea or phosphorylase CoASH or Asp or UDP-Glc

We conducted a survey of 300 RIT students and one of the survey questions was: “Did you use a ride-service (such as Uber/Lyft) during Fall 2024 semester?” Yes or No We are interested in developing a confidence interval with the survey results. Assume all assumptions have been met. Which statistical feature would we use and why?

Which point represents pKa1?  Which point represents a 50/50 mixture of the plus 1 and 0 charged species?  At which point does the major molecular species have a net charge of 0?  Which point represents the isoelectric point of this amino acid? At which point are there as many molecules with a net charge of 0 as have a net charge of -1?  

The high temperature for 50 cities are shown in the box plot below: Which temperature represents the 25th percentile?