Based on the Baltimore System, this is a class __ virus.

You are having a conversation with your friend and they tell you that there are four domains of life: eukaryotes, prokaryotes, archaea and viruses.  Do you agree with your friend?  Explain and provide evidence to support your answer. (six sentences max)

Use this prompt to answer questions 10-11. To better understand the importance of MRV’s glycoprotein in the virulence of the virus, researchers have produced mutant viruses with amino acid changes in glycoprotein and tested the virulence of the virus using the end-point dilution assay.   The wildtype virus (WT) has no mutations, C virions have mutations in the cytoplasmic tail, E virions have mutations in the ectodomain and F virions have mutations in the fusion peptide.  The results from the end-point dilution assay for each virus is shown below.

This virus’ capsid symmetry is ___.

Potato virus’ capsid symmetry is ____.

Explain how a mutation could decrease the virulence of the virus.  Recall this mutation is in the glycoprotein that mediates membrane fusion.  Answer this question by addressing the prompts A-D below. Which of the viruses is the least virulent?   Provide evidence from the end-point dilution assay to support your answer. (one sentence max) Which step(s) of the infection cycle is affected by this protein. (one sentence max) What is the role of this protein in membrane fusion (you must thoroughly describe the process of membrane fusion and the role of the glycoprotein in the process). (5 sentences max) How would this specific mutation negatively affect membrane fusion. (3 sentences max)

Use this prompt to answer questions 12-19. You discover a new virus that causes ulcers in the mouth and is transmitted through contact with infected saliva.  You name the virus Salivary Emission Ulcervirus, SEU for short.  SEU’s genome is single stranded DNA.  You are able to get an image using electron microscopy (left) and build a model of the virus (right).  After years of work, you find that SUE attaches to host cells using AB-1 as an attachment factor and YZ-2 as a receptor.  You use this information to better understand the tissue tropism of the virus.  You design an experiment to test multiple cell lines and determine if they can be infected by SEU.  Your results are below.

Based on the Baltimore System, SEU is a Class ___ virus.

In order for SEU to successfully infect a cell it must enter the cell via , triggered by the attachment of to the host’s proteins.

The viral genome can be packaged using… (choose all that apply).