You have generated recombinant plasmids using a pUC-based cl…

You have generated recombinant plasmids using a pUC-based cloning vector and a piece of a human gene. After applying a heat shock to your E. coli cells in the presence of the recombinant vector, you spread the cells onto an agar medium that includes X-gal and ampicillin. Which colonies will you choose to take to the next step, and why?