Deschenes Questions: 14-20 In class we discussed a genetic s…

Deschenes Questions: 14-20 In class we discussed a genetic screen to identify mutations in the cell biological machinery controlling vesicle formation and trafficking. A yeast cell harboring a temperature sensitive mutant, sec4ts, fails to secrete the enzyme invertase, an enzyme that degrades sucrose.   A.) How would you test the hypothesis that Sec4 is involved in vesicle trafficking? (1 points) B.) Describe how would you determine experimentally which step in vesicle trafficking is defective in the sec4ts mutant. (1 points)