You are interested in performing blue and white screening. You plate cells containing a vector with no insert in the presence of ampicillin, IPTG, and X-Gal. Which of the following would you expect to observe?
You are attempting to incorporate a mutation in a DNA sequen…
You are attempting to incorporate a mutation in a DNA sequence using PCR. To accomplish this, you design four primers. Which of the following primers would you use during PCR #3 shown in the image below?
Which of the following statements about PCR is FALSE?
Which of the following statements about PCR is FALSE?
You need to digest DNA with two enzymes that are functional…
You need to digest DNA with two enzymes that are functional under different conditions, and both require different buffers which are inhibitory on the other enzyme. What should you do?
DNA probes are generated by all of the following EXCEPT
DNA probes are generated by all of the following EXCEPT
Which of the following DNA purification techniques can poten…
Which of the following DNA purification techniques can potentially inhibit a downstream PCR?
What is the recommended daily intake (RDI) for iron that the…
What is the recommended daily intake (RDI) for iron that the midwife recommends when providing teaching to a pregnant woman?
Which enzyme can substitute for DNA ligase, if the both the…
Which enzyme can substitute for DNA ligase, if the both the vector and insert are modified appropriately first?
In inverse PCR,
In inverse PCR,
To introduce a His-tag onto a protein at the N-terminus, you…
To introduce a His-tag onto a protein at the N-terminus, you would need a forward primer that encoded