How does traditional sequencing differ from next generation sequencing?

DNA and RNA purity can be quantified by using

What is the Tm of the primer AGGCT?

Which of the following is NOT part of a Southern blot?

You are interested in performing blue and white screening. You plate cells containing a vector with no insert in the presence of ampicillin, IPTG, and X-Gal. Which of the following would you expect to observe?

You are attempting to incorporate a mutation in a DNA sequence using PCR. To accomplish this, you design four primers. Which of the following primers would you use during PCR #3 shown in the image below?

Which of the following statements about PCR is FALSE?

You need to digest DNA with two enzymes that are functional under different conditions, and both require different buffers which are inhibitory on the other enzyme. What should you do?

DNA probes are generated by all of the following EXCEPT

Which of the following DNA purification techniques can potentially inhibit a downstream PCR?