The following prokaryotic DNA is transcribed into RNA and th…

The following prokaryotic DNA is transcribed into RNA and the mRNA transcript is translated into protein.  Useful sequences: -35: 5’ TTGACA 3’, -10: 5’ TATAAT 3’ (Pribnow box), Shine Dalgarno sequence: 5’ AGGAGGU 3’ What will the sequence be for the protein translated from this mRNA?

You have generated recombinant plasmids using a pUC-based cl…

You have generated recombinant plasmids using a pUC-based cloning vector and a piece of a human gene. After applying a heat shock to your E. coli cells in the presence of the recombinant vector, you spread the cells onto an agar medium that includes X-gal and ampicillin. Which colonies will you choose to take to the next step, and why?