Consider a hypothetical scenario in which each of the follow…

Consider a hypothetical scenario in which each of the following oligopeptides is capable of folding into a stable native state in aqueous solution. When we measure the rates at which each of these oligopeptides folds starting from the denatured (i.e. unfolded) state, we find that three of them appear to fold as single populations, i.e. in these three cases, all copies of the oligopeptide appear to fold at approximately the same rate. In contrast, with the fourth oligopeptide, there appears to be two distinct populations: some copies of the oligopeptide fold rapidly, while other copies fold much more slowly. Given only this information and the sequences shown below, identify the oligopeptide sequence that is most likely to exhibit two distinct folding populations.

Consider a globular protein whose native (i.e. folded) state…

Consider a globular protein whose native (i.e. folded) state is: (a) monomeric and (b) thermodynamically stable in aqueous solution at room temperature and pH 7. Given what we have discussed in the lectures, which of the following changes would be least likely to cause the protein to unfold?