A soluble, cytosolic protein is expressed in E. coli. After centrifugation, SDS-PAGE locates the protein in the pellet. Which chemical or technique would be best to solubilize this protein?

What is the molecular weight of the band found in Lane 2 below?

Which of the following can be tried to prevent inclusion bodies in from forming in E. coli?

In the SDS-PAGE gel from an affinity column below, Lane 2 shows the

The gel below shows an induced cell lysate of a his-tagged protein, with the pre-induced (lane 1), insoluble (lane 2), and soluble (lane 3) fractions. What technique should you use first to purify this protein?

Protein-protein and protein-drug interactions can be studied on a massive scale using

Two proteins elute in fractions 30 and 40 of a size exclusion column on their own. You mix them in equal molar ratios and run them over the same column. What would you expect if they interact?

A protein that can be acetylated has five domains (A through E). Researchers deleted these domains in the western blot of an SDS-PAGE below. From this data, you can conclude that

What is the molecular weight of the band found in Lane 3 below?

A technique that is useful in trying to identify new protein-protein interactions from a homogenate is