Which of the following is an advantage of in vitro protein expression?

Prior to running your sample in isoelectric focusing, you must add

A calibration curve using protein standards is useful in

An experiment was performed to induce and purify the protein X. In the gel below, Lane 1 shows non-induced cells; Lane 2 shows induced cells; Lane 3 is purified MBP-X; Lane 4 is the protein after TEV cleavage; Lane 5 is the flowthrough after the cleaved sample has been run through an amylose column; Lane 6 is the purified TEV protease. Which band represents the protein of interest?

The beads in a cation exchanger are

Which technique uses phenyl isothiocyanate to bind to N-terminal amino acids?

In cation exchange chromatography, when the salt gradient is increased from 0 to 100%,

When running size exclusion chromatography, you get two peaks merged together as seen below. Which of the following is the most appropriate solution for separating the two peaks?

In western blotting, the enzyme is typically conjugated to the

The image below, which illustrates an increased size on the gel after the addition of Protein 2, is demonstrating