This drug acts as both a stimulant and a psychedelic.  It produces an energizing effect that distorts both physical nad coginitive sensations and it may impair memory. It works by affecting a neuron’ ability to use the chemical serotonin.  It can sometimes result in too much water in the blood which can cause brain swelling.  

Which of the following is true about protein domains?

 Importin and exportin are proteins involved in. . .

You have two beakers as depicted below.  Both are split in the middle and contain a semipermeable membrane. Both also contain a solute which cannot cross the semipermeable membrane by itself; the amount of solute is different on both sides of the beaker. Beaker #2 contains transporters embedded in the semi-permeable membrane that will allow the solute to pass.    You leave the beakers this way for a few hours and then come back to observe the results. What is most likely to happen?  

Methods in Cell Biology Short Answer Section (10 pts)

SA2c. (5 pts) Describe how CHIP28 (aquaporin) was shown experimentally to be a water channel.  Include a description of both the procedure and the result. (Your answer should include 5 steps).  1. 2. 3. 4. 5.

Membranes Short Answer Section (10 pts)

Which type of protein sorting involves SRP?

Researchers interested in studying possible interactions between lamellipodin (Lpd) and Mena performed the experiment below.  Lpd-FP4-2Mito is a fusino protein consisting of Lpd, the FP4 domain which has been shown to bind to Mena, and the 2Mito domain which has been shown to bind to the mitochondria.  The Lpd-Pro-Mito fusion protein is similar except that the FP4 domain has been replaced with a bunch of prolines that don’t really do anything other than act as a spacer.    Look at the immunofluorescence images and indicate which of the following was the most important result of this experiment.

Part II: Short Answer (6 questions, 5-10 points each) 40 points