Which technique can be used to analyze tissue-specific mRNA (and thus protein) levels in a cell?

When designing PCR primers that contain restriction sites, one should always ensure that

Which of the following techniques does NOT involve DNA or RNA hybridization?

The image below shows a

The pGEM-T vector comes linearized, with T overhangs. This allows for

Which is NOT an advantage of ligation independent cloning?

In the 1970s, researchers could use each of the following tools to study DNA EXCEPT

In bisulfite sequencing,

Which DNA purification technique involves purifying the sample using an ultracentrifuge?

You are attempting to incorporate a mutation in a DNA sequence using PCR. To accomplish this, you design four primers. Which of the following primers would you use during PCR #2 shown in the image below?