Based on the diagram below answer the following questions:  …

Based on the diagram below answer the following questions:   a) What enzyme mechanism is depicted in the figure above? b) What are the names of the three amino acids in the catalytic site, in the correct order from left to right c) How does histidine from the catalytic triad act in step 2? d) How does histidine from the catalytic triad act in step 3? e) Which amino acid acts as a nucleophile ? f) Which steps of the mechanism above correspond to the burst phase, and which steps correspond to the steady-state phase, as shown in the graph below?  

The illustration below shows several oxygen-dissociation cur…

The illustration below shows several oxygen-dissociation curves for hemoglobin. Assume that curve 3 corresponds to hemoglobin with physiological concentrations of CO2 and 2,3-BPG at pH 7. Which curves (chose the curve number that best fits) represent each of the following perturbations?    a)  Decrease in CO2 b)  Increase in 2,3-BPG c)  Increase in pH d)  Loss of quaternary structure