8. You use Agrobacterium to “break” genes at random in Arabi…

8. You use Agrobacterium to “break” genes at random in Arabidopsis, and harvest the transformed seeds. Note: you have already done the Agro transformation now. In the space below, outline how you will screen for an ABA mutant among your transformed offspring. What will you manipulate, experimentally? (1-2 sentence limit)   What will you use as a control? (1 sentence limit)   What phenotype are you looking for in your transformed seeds, compared to wild-type? Be specific. (1 sentence limit)  

Which of the following answers most accurately summarizes st…

Which of the following answers most accurately summarizes statistically significant (P=0.05) differences in the data in the table below? Table: Z-test analyses of the effects of gibberellic acid (GA) and abscisic acid (ABA), each at two different concentrations, on seed germination of two species after 7 days. 100 seeds of each species were in each treatment group. Name of Plant Species Di H2O 10-6 M GA 10-4 M GA 10-6 M ABA 10-4 M ABA Geranium germination % 96 81 84 88 24 Geranium Z-value 1.48 1.23 0.97 3.89 Geranium Z-value probability (p-value) 0.14 0.22 0.33

4. The soil that our plant was growing in was low on Fe2+, a…

4. The soil that our plant was growing in was low on Fe2+, and the plant is now iron-deficient, shown below.  It turns out that the soil is low on Fe2+ because it is acidic. Briefly explain why acidic soils are more likely to be nutrient-poor than pH-neutral or alkaline soils. (2-4 sentence limit) Extra credit – 1pt Is this a deficiency in a mobile element or an immobile element? Explain how you can tell. (1-2 sentences)