You wish to amplify a piece of genomic DNA (it is not protein-coding) and ligate it into a vector using EcoRI and BamHI. What should the primers look like?

Which of the following enzymes can be used to join together two fragments of DNA?

Bacteria that take up DNA from the environment are said to be

A primer has a Tm of 50 °C, but you attach a restriction enzyme cut site which increases it to 55 °C. Which of the following is TRUE?

Which of the following transformation techniques provides a time constant, which indicates if transformation was successful?

You wish to optimize a PCR reaction, and perform the experiment at several different temperatures (from ~40-60 °C). Which step of PCR is was modified?

Which technique involves linking primers directly to beads to allow for easy retrieval, analysis, and to reduce contamination?

In the pedigree below, which represents the dot blot you’d expect of the child?

In Maxam-Gilbert sequencing,

Three DNA samples undergo VNTR analysis, as shown below. Arrows denote digestion sites. Which of these three individuals is represented in Lane 2 on the gel to the right?