You use bacterial two-hybrid to study proteins A and B below. If you randomly mutate protein A at the position noted,

Mass spectrometry can be used to determine all of the following EXCEPT

Why is a solubility test important before ion exchange chromatography?

Two proteins elute in fractions 30 and 40 of a size exclusion column on their own. You mix them in equal molar ratios and run them over the same column, and you only see peaks in fractions 30 and 40. What would you conclude?

What is the purpose of adding EDTA to a lysate?

When expressing proteins such as insulin, yeast may be more advantageous than E. coli because

A technique that is useful in identifying RNA-protein interactions is

Why does the phage vector system have lower levels of background expression than the Ptac system?

Which of the following is TRUE regarding purifying proteins using the ammonium sulfate precipitation?

You purify 100 ml of protein, and the buffer has 200 mM NaCl. How many dialyses will you need to perform to reduce the salt concentration to below 10 mM, if dialyzing into 1 L of buffer with no salt?