The gel below shows an induced cell lysate of a his-tagged protein, with the pre-induced (lane 1), insoluble (lane 2), and soluble (lane 3) fractions. What technique should you use first to purify this protein?

Protein-protein and protein-drug interactions can be studied on a massive scale using

Two proteins elute in fractions 30 and 40 of a size exclusion column on their own. You mix them in equal molar ratios and run them over the same column. What would you expect if they interact?

A protein that can be acetylated has five domains (A through E). Researchers deleted these domains in the western blot of an SDS-PAGE below. From this data, you can conclude that

What is the molecular weight of the band found in Lane 3 below?

A technique that is useful in trying to identify new protein-protein interactions from a homogenate is

Which of the following is an advantage of in vitro protein expression?

Prior to running your sample in isoelectric focusing, you must add

A calibration curve using protein standards is useful in

An experiment was performed to induce and purify the protein X. In the gel below, Lane 1 shows non-induced cells; Lane 2 shows induced cells; Lane 3 is purified MBP-X; Lane 4 is the protein after TEV cleavage; Lane 5 is the flowthrough after the cleaved sample has been run through an amylose column; Lane 6 is the purified TEV protease. Which band represents the protein of interest?