Several years later, Miss C is thinking about trying to start a family. She sees her GP, who arranges thyroid function tests.   Patient Result Reference Range Free T4                   60 mmol/L (70-140 mmol/L) TSH                       9 mIU/L (0.3-4.7 mIU/L)

What is a potential complication of untreated gonorrhoea for this patient?

In single-cell RNAseq analysis, what is the use of Unique Molecular Identifier (UMI)?  

Which of the following is the most appropriate advice to provide to Mr A relating to the use and storage of Ozempic? https://www.medicines.org.uk/emc/product/9748

Ms. J decided to use diagnostic (end-point) PCR to determine if an insect carry a mutation of an ion channel gene that mediates insecticide resistance. She then decided to also check if the insect have a mutation of another gene. What PCR “ingredient” would be different between these two tests?

John collected 9 samples of nematodes treated with different chemicals. He planned to examine differences among their transcriptomes by Illumina sequencing. However, he forgot to include index sequences for his library preparation in the single sequencing run for multiplexing. What problem will John face?

In the CRISPR system, what is the key difference between cas1 and cas9 proteins? 

In Western blot, indirect detection using an unlabeled primary antibody followed by a labelled secondary antibodies increases the detection sensitivity.

Sequencing a genome of 200Mb, using the Illumina paired-end 100bp, and yielding 50 million reads, gives a coverage of:

Which of Miss C’s presenting complaints most strongly suggests an alternative diagnosis to urinary tract infection?