Which method would be best for purifying soluble proteins?

You run an enzyme through gas chromatography. You would expect to see

SDS-PAGE requires sodium dodecyl sulfate to be added to the sample because it

If you inject a human protein into a goat, the antibodies made against the human protein will be

An experiment was performed to induce and purify the protein X. In the gel below, Lane 1 shows non-induced cells; Lane 2 shows induced cells; Lane 3 is purified MBP-X; Lane 4 represents the protein after TEV cleavage; Lane 5 represents the flowthrough after the cleaved sample has been run through an amylose column; Lane 6 represents the purified TEV protease. What is the molecular weight of the free MBP tag?

You are reading a paper which shows an image of a gel, and concludes that protein A is expressed in higher amounts than protein B based on band intensity. You could confirm this by using the

Which is TRUE regarding SDS-PAGE and western blotting?

What pH must you use to purify a protein with a pI of 4.2 via cation exchange chromatography?

A technique that involves crosslinking proteins to DNA, purifying the fragments with antibodies, and determining the protein-DNA binding sites is called

You perform a western blot, but skip the blocking step. This will result in