A protein dimer with subunits connected by disulfide bridges is run through an SEC column and elutes in fraction 20. After 100% successful treatment with a reducing agent, the protein is rerun over the same column. Which of the following is TRUE?
Which of the following antibodies would be best to use for a…
Which of the following antibodies would be best to use for an ELISA where specificity is very important?
Which technique is most useful for obtaining specific mutant…
Which technique is most useful for obtaining specific mutants of an existing protein?
Capillary tube gels are used in
Capillary tube gels are used in
All of the following can be used to sequence proteins EXCEPT
All of the following can be used to sequence proteins EXCEPT
Which is NOT a technique for lysing cells?
Which is NOT a technique for lysing cells?
Which technique involves drying a protein sample out under v…
Which technique involves drying a protein sample out under vacuum?
Guanidine-HCl and urea
Guanidine-HCl and urea
The proteasome degrades ubiquitinated proteins. You treat ce…
The proteasome degrades ubiquitinated proteins. You treat cells with a proteasome inhibitor (+ lane), and visualize with an anti-ubiquitin antibody. What would be the expected results of inhibitor treatment?
Why aren’t native gels typically used to determine the molec…
Why aren’t native gels typically used to determine the molecular weight of proteins?