An Ames test was carried out on a suspected mutagen, which w…

An Ames test was carried out on a suspected mutagen, which we will call mutagen A. In each case, one million bacteria were plated on media that lacked histidine. The following numbers of colonies were observed to grow.                                     Trial    Control            With mutagen A                                     1           7                              43                                     2           9                             19                                     3           4                              27                                     4           5                              33                                     5           7                              44   You don’t need to conduct a t-test now, but you can probably guess what the results would be. Which of the following conclusions would you draw?

The restriction enzyme EcoRI recognizes the sequence: 5′-G/A…

The restriction enzyme EcoRI recognizes the sequence: 5′-G/AATTC-3′ 3′-CTTAA/G-5′ It cuts between the G and A as noted by the slashes. Following the digestion of chromosomal DNA and vector DNA with EcoRI, let’s suppose that one fragment of chromosomal DNA gets inserted into a vector. No DNA ligase has been added. If both of the sticky ends of the chromosomal DNA fragment are binding to the sticky ends of the vector, what is holding these two pieces of DNA together?

One method of gene cloning involves the insertion of a fragm…

One method of gene cloning involves the insertion of a fragment of chromosomal DNA into a vector, such as a plasmid. During this procedure, three outcomes are possible, as shown below: The vector on the left is a recircularized vector, the one in the middle is a recombinant vector that carries a gene of interest (the gene you want to clone), and the one on the right is a recombinant vector that has any other random fragment of chromosomal DNA. Answer the following four questions that pertain to this type of cloning.

Please use the case study below to answer questions 90-95. C…

Please use the case study below to answer questions 90-95. Client History 74 year old client diagnosed with stage 4 lung cancer with metastasis to the brain and liver. Client lives with son and his family, who are his main caregivers at home. Clients advanced directive states that he does not want CPR, intravenous fluids, antibiotics, a feeding tube, or hospitalization at the end of life. The son is his health care proxy.   Nurses Note Client sitting in recliner. Awake and confused, and insists he needs to go see his wife, who has been deceased for 15 years. Son states is very concerned because the client has become more agitated over the past two days and is not sleeping at night. Client skin is hot and pale, oral mucosa dry. Breath sounds diminished throughout with coarse rhonchi on the right. Productive cough with large amounts of thick, white sputum. Client reports terrible pain in his back that is not being relieved by his current pain medication. Taking sips of water, but refuses food and reports having no appetite.   Vital Signs Blood Pressure 106/54 Heart Rate 90 irregular Temperature 100.6 F (38.22 C) Respiratory Rate 18 SpO2 92% on 2L oxygen continuously via NC Question: The priority education for the family would include which of the following?