Most centrifuges use a rotor that angles the tubes because

What is the extinction coefficient of a protein with a concentration of 1.25 μM and absorbance of 0.45?

The Lowry assay is advantageous over the Bradford assay because

How is cDNA generated?

What would you expect the absorbance of a sample to be if the transmittance was 63.42%?

The Belmont Report requires “beneficence”. This refers to

How do you prepare 450 ml of 40 mM Tris (Mw = 121.14)?

A protein has a concentration of 50 µM, and 4 tryptophans (ε = 5500) and 2 tyrosines (ε = 1490). What is its absorbance?

You wish to study the effects of substrate concentration on enzyme rate. For the experiment, you alter the substrate concentration and measure the change in enzyme rate, while making sure the enzyme concentration is constant in each sample. Which is the dependent variable?

Using the standard curve below, what is the concentration of a sample that gives an absorbance of 0.5 when 5 microliters of sample are assayed?