Which of the following is FALSE about protein induction?
High pressure liquid chromatography (HPLC) is the best choic…
High pressure liquid chromatography (HPLC) is the best choice for separating all of the following for downstream use EXCEPT
You analyze a protein and obtain the results shown below. Wh…
You analyze a protein and obtain the results shown below. What is TRUE about this gel?
Peak 3 below from an ion exchange column shows
Peak 3 below from an ion exchange column shows
A protein is acetylated at lysine 255 (K). Researchers mutat…
A protein is acetylated at lysine 255 (K). Researchers mutated the protein in the western blot of an SDS-PAGE below. What can you conclude from this figure?
Which of the following is FALSE regarding purifying proteins…
Which of the following is FALSE regarding purifying proteins using the ammonium sulfate precipitation?
You wish to express a protein expression system that is very…
You wish to express a protein expression system that is very fast, inexpensive, and has established protocols that have been perfected over the past few decades, rather than explore a newer system. Which expression system would you choose?
Why are proteins stored in a buffer with dithiothreitol or β…
Why are proteins stored in a buffer with dithiothreitol or β-mercaptoethanol?
You purify a protein with an anion exchange chromatography a…
You purify a protein with an anion exchange chromatography and elute it with 1 M NaCl. You take this eluate and load it directly onto a cation exchange column, but the protein was found only in the flowthrough and not the elution. What was the cause of this?
A pregnancy test is equivalent to which of the following tec…
A pregnancy test is equivalent to which of the following techniques?