When running size exclusion chromatography, you get two peaks merged together as seen below. Which of the following is the most appropriate solution for separating the two peaks?

In western blotting, the enzyme is typically conjugated to the

The image below, which illustrates an increased size on the gel after the addition of Protein 2, is demonstrating

You perform an SDS-PAGE and observe a band at the expected molecular weight of your target protein. What technique would be best to confirm this?

Which of the following polyacrylamide gel reagents is NOT properly matched up with the correct function?

Ammonium sulfate precipitation

Why would the diameter of the beads affect SEC resolution?

You wish to express a human protein that is properly folded, contains cleaved signal peptides, proper glycosylation, and other processing modifications. Which expression system would you choose?

Which statement about chromatography is TRUE?

In which expression system does E. coli RNA polymerase enzyme transcribe the gene of interest on the plasmid into mRNA?