In anion exchange chromatography, when the salt gradient is increased from 0 to 100%,

An experiment was performed to induce and purify the protein X. In the gel below, Lane 1 shows non-induced cells; Lane 2 shows induced cells; Lane 3 is purified MBP-X; Lane 4 is the protein after TEV cleavage; Lane 5 is the flowthrough after the cleaved sample has been run through an amylose column; Lane 6 is the purified TEV protease. What is the molecular weight of the protein of interest?

Which of the following polyacrylamide gel reagents is NOT properly matched up with the correct function?

In native gel electrophoresis, proteins A and B migrate to the same position on the gel. Which of the following is TRUE?

A device that uses a piston to put high pressure on cells to lyse them is a

To determine the number of domains a protein has, you could use

An anti-mouse monoclonal antibody to a nonlinear conformational epitope would be useful in all of the following techniques EXCEPT

In size exclusion chromatography, C-100 grade beads are larger than S-100 beads. Which statement is TRUE?

Why is a gradient of 0 to 100% salt used in ion exchange chromatography?

Which of the following techniques can be used to separate proteins based on pI?