A DNA fragment of unknown sequence is divided into four port…

A DNA fragment of unknown sequence is divided into four portions and mixed with all the elements necessary to synthesize the complementary strand according to the Sanger sequencing method. To each of these four mixtures a different dideoxynucleotide (a modified base) is added in addition to the normal nucleotides.  Each mixture is then separated by electrophoresis and the gel shown below is obtained. What is the sequence of the original strand of DNA?  Note: The A, T, C, G labels at the top of each lane refer to the dideoxynucleotide that was added in each reaction mixture.

Students who take this exam are expected to NOT engage in an…

Students who take this exam are expected to NOT engage in any form of academic dishonesty. This includes using information from any outside resources such as textbook, internet, and others. You are, however, allowed a “cheat sheet”- one side of one-half of a standard 8.5 x 11 piece of paper with HAND-WRITTEN notes. Hold your “cheat sheet” up to your webcam so that it is visible for about three seconds (failure to do so may result in a score of 0 for this exam).  You may also use a calculator (not a smart phone) and one blank piece of scratch paper.  Indicate your willingness to NOT engage in academic dishonesty by indicating your response below. Note: you must choose option “a” in order to receive credit for this exam.