What conclusions could you draw from this peak from a size exclusion column?

A protein was run on both an SDS-PAGE and native gel, as shown below, with the addition of a drug (+) that disrupts protein structure. Which of the following is TRUE?

Which of the following can be used to determine a protein’s molecular weight, or to detect protein interactions with metals by analyzing weight differences in the holo vs. apo state?

Which would be the best choice for studying protein-protein interactions or protein modifications?

Which of the following techniques can be used to identify where a protein binds in a DNA sequence?

What is the purpose of Tween-20 in the western blot washes?

What is the molecular weight of the band found in Lane 4 below?

In chromatography, the stationary phase’s function is to

A soluble, cytosolic protein is expressed in E. coli. After centrifugation, SDS-PAGE locates the protein in the pellet. Which chemical or technique would be best to solubilize this protein?

What is the molecular weight of the band found in Lane 2 below?