Human insulin that is used to treat diabetic patients is commonly made in bacteria. When insulin is made in bacteria, why are the A and B chains are linked to β-galactosidase?
Which curve (A or B) is more aggressive?
Which curve (A or B) is more aggressive?
As described in your textbook, one way to determine if a seg…
As described in your textbook, one way to determine if a segment of chromosomal DNA has been inserted into a vector is to put XGal in the growth media. Following overnight growth,
Which of following types of genetic sequences would come fir…
Which of following types of genetic sequences would come first in a gene?
A primer used in dideoxy DNA sequencing is 20 nucleotides lo…
A primer used in dideoxy DNA sequencing is 20 nucleotides long and has the following sequence: 5’-GGATCCATGACTAGTCCGAC-3’. A segment of DNA is cloned into a vector and then the vector DNA is denatured and subjected to dideoxy DNA sequencing method. Below is the DNA sequence from a region of the vector. The primer-annealing site is shown in bold and underlined. 3-CCCGATCGGCCTAGGTACTGATCAGGCTGAATGACT-5’ Based on the sequence above, what would be the size(s) of the band(s) (i.e., the number of nucleotides in each band) in which dideoxyT had been added to the sequencing reaction?
Metagenomics is:
Metagenomics is:
The identification of a stop codon for a particular gene is…
The identification of a stop codon for a particular gene is an example of
In the biotechnological production of human insulin (Humulin…
In the biotechnological production of human insulin (Humulin), how are the A or B chains separated from beta-galactosidase?
You can use tandem mass spectrometry to:
You can use tandem mass spectrometry to:
The restriction enzymes shown below cut DNA in the following…
The restriction enzymes shown below cut DNA in the following manner. The slashes indicate where cutting occurs. BamHI G\GATCC CCTAG\G EcoRI G\AATTC CTTAA\G HaeII GGCGC\C C\CGCGG PstI CTGCA\G G\ACGTC Which of these enzymes would generate sticky ends that would be the least stable when they bind to complementary sticky ends?