Which micropipette should be used for the most accurate transfer of 50 microliters?
A 150 kDa protein would consist of approximately ______ amin…
A 150 kDa protein would consist of approximately ______ amino acids.
You analyze a DNA sample which gives an A260 of 1.56 and an…
You analyze a DNA sample which gives an A260 of 1.56 and an A280 of 0.98. What is the concentration of this DNA sample, and is it sufficiently pure?
How do you prepare 600 ml of 50 mM Tris (Mw = 121.14)?
How do you prepare 600 ml of 50 mM Tris (Mw = 121.14)?
You want to load DNA onto a gel which has 40 µl wells. How m…
You want to load DNA onto a gel which has 40 µl wells. How much of a 5X stock of sample loading buffer should be mixed with the DNA to fill up the well?
You add 1000 microliters of Folin reagent to a lysate sample…
You add 1000 microliters of Folin reagent to a lysate sample and read its absorbance in the spectrophotometer, which gives a reading of 0.3 compared to a blank. This is a(n) ________ measurement.
Which of the following would you use for accurately dispensi…
Which of the following would you use for accurately dispensing a sample in a single dispense, and is considered “to deliver”?
Which type of inhibitor greatly reduces the Vmax of a reacti…
Which type of inhibitor greatly reduces the Vmax of a reaction, and can’t be overwhelmed by substrate?
You wish to study the effects of substrate concentration on…
You wish to study the effects of substrate concentration on enzyme rate. For the experiment, you alter the substrate concentration and measure the change in enzyme rate, while making sure the enzyme concentration is constant in each sample. Which is the control?
Which type of inhibitor typically binds to the enzyme’s acti…
Which type of inhibitor typically binds to the enzyme’s active site?