Which of the following is FALSE about protein induction?
A technique that allows you to determine the thermodynamics…
A technique that allows you to determine the thermodynamics of protein-protein interactions is
To identify a protein on a 2D gel, you can perform all of th…
To identify a protein on a 2D gel, you can perform all of the following EXCEPT
You express a protein using both the Ptac and phage systems…
You express a protein using both the Ptac and phage systems in E. coli and run the pre-expressed and induced samples on a gel (below). What can you conclude?
You are studying a tetramer that consists of a 20 kDa homodi…
You are studying a tetramer that consists of a 20 kDa homodimer and a 30 kDa homodimer. You would expect to see ______ band(s) on a native gel and ______ band(s) on an SDS-PAGE.
What is the disadvantage to fusing a target protein to GST?
What is the disadvantage to fusing a target protein to GST?
In anion exchange chromatography, when the salt gradient is…
In anion exchange chromatography, when the salt gradient is increased from 0 to 100%,
An experiment was performed to induce and purify the protein…
An experiment was performed to induce and purify the protein X. In the gel below, Lane 1 shows non-induced cells; Lane 2 shows induced cells; Lane 3 is purified MBP-X; Lane 4 is the protein after TEV cleavage; Lane 5 is the flowthrough after the cleaved sample has been run through an amylose column; Lane 6 is the purified TEV protease. What is the molecular weight of the protein of interest?
Which of the following polyacrylamide gel reagents is NOT pr…
Which of the following polyacrylamide gel reagents is NOT properly matched up with the correct function?
In native gel electrophoresis, proteins A and B migrate to t…
In native gel electrophoresis, proteins A and B migrate to the same position on the gel. Which of the following is TRUE?