In the biotechnological production of human insulin (Humulin), how are the A or B chains separated from beta-galactosidase?

You can use tandem mass spectrometry to:

The restriction enzymes shown below cut DNA in the following manner. The slashes indicate where cutting occurs. BamHI                         G\GATCC                                     CCTAG\G   EcoRI                          G\AATTC                                     CTTAA\G        HaeII                           GGCGC\C                                    C\CGCGG   PstI                             CTGCA\G                                    G\ACGTC Which of these enzymes would generate sticky ends that would be the least stable when they bind to complementary sticky ends?

Hematopoietic stem cells (blood cells) in your body are

Trinucleotide repeat expansion is caused by

When a fragment of chromosomal DNA is inserted into a vector, how many covalent bonds are made via DNA ligase? 

Work in a forensic lab and you need to do a paternity test. You would:

An Ames test was carried out on a suspected mutagen, which we will call mutagen A. In each case, one million bacteria were plated on media that lacked histidine. The following numbers of colonies were observed to grow.                                     Trial    Control            With mutagen A                                     1           7                              43                                     2           9                             19                                     3           4                              27                                     4           5                              33                                     5           7                              44   You don’t need to conduct a t-test now, but you can probably guess what the results would be. Which of the following conclusions would you draw?

The restriction enzyme EcoRI recognizes the sequence: 5′-G/AATTC-3′ 3′-CTTAA/G-5′ It cuts between the G and A as noted by the slashes. Following the digestion of chromosomal DNA and vector DNA with EcoRI, let’s suppose that one fragment of chromosomal DNA gets inserted into a vector. No DNA ligase has been added. If both of the sticky ends of the chromosomal DNA fragment are binding to the sticky ends of the vector, what is holding these two pieces of DNA together?

One strategy to produce a protein in the milk of a cow is to place the coding sequence of the gene of interest next to a __________ and then inject the gene into a _________.