A pregnancy test is equivalent to which of the following techniques?

You are working with a protein that is polyubiquitinated (a variable number of ubiquitins), which you visualize with an anti-ubiquitin antibody. You treat the protein with an enzyme that cleaves ubiquitin from proteins and degrades it (+ lane). What would be the expected results?

A blue native PAGE is advantageous over a standard native gel because it

You’re working with an MBP-p53 fusion protein, which is linked by covalent peptide bonds. You would expect to see ______ band(s) on SDS-PAGE, and ______ band(s) on a native gel.

A protein has a pI of 5, and is in a buffer at pH 7. What type of column should it bind to?

Which of the following would be best to study the glycosylation state of a protein?

In the phage vector induction system,

You attempt to express a large protein using yeast cells, but the protein did not properly fold with this system. Which of the following is the appropriate next step for achieving successful protein expression?

You use bacterial two-hybrid to study proteins A and B below. If you randomly mutate protein A at the position noted,

Mass spectrometry can be used to determine all of the following EXCEPT