You are studying a microRNA, miRNA1, and decide to PCR ampli…

You are studying a microRNA, miRNA1, and decide to PCR amplify its entire coding region and add it to the multiple cloning site (MCS) of the plasmid shown below (the intended “insert”). miRNA1 is 22 nucleotides in length. After attempting your cloning reaction, you transform the plasmid into bacteria and grow the bacteria with both X-Gal and IPTG on their growth medium. Select all correct results you should expect when attempting this cloning procedure. -The MCS is a cluster of restriction endonuclease cut sites used to add DNA to the plasmid via restriction-ligation cloning). -“lacZ” indicates a functional lacZ gene with the normal promoter and operator from the lac operon. This gene is functional with a small MCS added to it in-frame (as shown above), but any frameshift OR the addition of >200 bp of DNA within the MCS abolishes lacZ function. -X-Gal is a substrate of the lacZ gene product that turns bacteria from colorless/white to blue when it is metabolized. -IPTG is an allolactose analog that prevents LacI from binding the lac operator. -Ampicillin is a commonly used antibiotic that kills all bacteria that do not express an AmpR resistance gene (which is also found on the plasmid).

You create a stable transgenic line of flies with an overexp…

You create a stable transgenic line of flies with an overexpression transgene that causes Ultrabithorax (Ubx) overexpression in the 2nd thoracic segment of a fly (“T2” in brown). Below is shown a wild type fly and a summary of some key genetic interactions that usually occur. Evaluate whether each statement below represents a logical outcome of this modification to T2 (true or false). Antp transcription will be inhibited in T2. Antp transcription will be released from inhibition in T3. Antp products will switch from promoting wing fate to haltere fate. T2 will grow a haltere instead of a wing. T3 will grow a wing instead of a haltere.

Which of the following statements about epigenetic inheritan…

Which of the following statements about epigenetic inheritance are true? Select true or false for each statement.  Imprinting occurs when males and females downregulate unique sets of genes by promoter methylation. Methylation resulting from imprinting is erased and reset in the germ line each generation. Some methylation events have been shown to demonstrate significant heritability. Following DNA replication, hemimethylated DNA is targeted by DNA methyltransferase 1 (DNMT1) to restore methylation patterns to the newly synthesized strand. A highly condensed chromatin state (heterochromatin) can “spread” on a chromosome, causing descendant cells to sometimes exhibit phenotypic differences because one or more genes are no longer expressed as strongly.