At the beginning of anaphase, there is a surge of Ca2+. Foll…

At the beginning of anaphase, there is a surge of Ca2+. Following this,  contributes to the degradation of by adding ubiquitin molecules to the protein. Once the protein is degraded, , the protease that it regulates, is released. This protease then cleaves , which is bound to sister chromatids, holding them together until anaphase. This final step triggers anaphase separation of the sister chromatids.