A viable plate has a count of 125 colonies produced from 0.125 mL of a 10-4 dilution of the original sample. The original sample contains _____ CFU/mL.

The inoculation loop must be sterilized _____ when creating a streak plate.  Check all that apply.

After using loops, swabs, toothpicks, spreaders or any other equipment to transfer cultures, immerse the contaminated equipment in ______.

After preforming a serial dilution to 105, the results  of the dilution plating were 45 colonies on the plate diluted to 105 . Calculate the CFU/ml of the original sample.  Remember to answer in scientific notation.

A 27-year old white female presented at the walking clinic of her local physician on August 15. On physical exam, the patient had a fever of 38.5C. She appeared fatigued, had tender joints, and complained of a headache, a stiff neck and a backache. The physician noticed a circular “rash” about 5 inches in diameter, with a bright red leading edge and a dim center in the form of a “bull’s eye”. The physician noted an irregular heart beat. The patient complained of lack of ability to concentrate. The patient gave the following history: She is a graduate student in the wildlife program at the university in town. She was in the field for three weeks in Wisconsin during the months of May and June. She tracks small mammals in the field and studies their behavior. It had been a warm, wet spring and she complained of a large number of biting flies, mosquitoes and ticks in the area. She felt well until about 2 weeks after returning to her home. Since that time, many of her symptoms had progressed. She finally found that she could take it no more. Gram staining revealed the following image: What is the appearance of the pathogen to be under the microscope using a gram stain? (focus on the larger pink microorganism)

An infection acquired in a hospital is referred to as a(n) _____ infection.

In the growth curve pictured below, at what point is the growth rate less than the death rate?

What is catalase?

In the figure below, line “D” points to the microscope’s

Plates with _____ colonies per plate are used to calculate CFUs.