A patient is being tested to determine whether he has the factor V Leiden mutation by PCR-RFLP analysis. PCR is performed on the patient’s DNA and cut with HindIII. The mutation-positive control sample had a single band of expected size, and the mutation-negative control sample had a slightly larger band. The patient’s specimen had two bands, one that was the same size as the mutation-positive control band and the other the same as the mutation-negative control. Which of the following is the correct interpretation of these results?

Which of the following laboratory methods used to detect mutations relies on differences in dissociation of double-stranded DNA into single-stranded DNA at different points in a gel?

A melt curve analysis was performed to genotype hepatitis C virus (HCV), which has been identified in a patient. When the derivative of fluorescence was plotted versus the temperature for the patient’s isolate as well as for a known genotype of HCV, the two sequences had identical peaks and thus the same Tm. Which of the following is the correct interpretation of these results?

A medical laboratory scientist performs fluorescence in situ hybridization (FISH) on interphase chromosomes using a probe to chromosome 21. In all cells examined, two signals were seen. These results are interpreted as:

A protein with the sequence DAILMNCST is mutated such that amino acids L and M are deleted. How is this mutation noted by accepted nomenclature?

Which of the following is involved in connecting the centromere to the spindles during chromosome segregation in mitosis?

Which of the following types of chromatin are open and actively involved in gene expression (transcription)?

Two enzymes of unknown concentrations, A and B, act on the same substrate, S. The initial velocity (V0) of the reaction catalyzed by Enzyme B is 60% of the V0 for Enzyme A when both enzymes are assayed at = 100 mM. Enzyme A has a Km of 20 mM, and enzyme B has a Km of 100 mM. Both Enzyme A and B have a Vmax of 120 mM/s. (6 pts)   A) What is the initial velocity (V0) for Enzyme A with its kinetic parameters and provided? (3 pts)   B) From these results, can you draw the conclusion on whether Enzyme A’s kcat is higher/lower than or equal to that of Enzyme B? Explain. (3 pts)

A nucleosome consists of DNA and which of the following combinations of histone proteins?

Seyfang Questions: 20-33 In which compartment of the cell does glycolysis occur? (1.5 pts)